Research

DMS sophomore to study if tiny algae grow calcium carbonate crystals

A supply grant from UConn's Office of Undergraduate Research (OUR) will test whether cyanobacteria could assist with removing carbon dioxide

Evelyn Lewis glances at the well plates full of colorful slime in Prof. Visscher’s lab and smiles. The life thriving in there is invisible to the naked eye, but she knows how to keep the microscopic critters happy. For almost a year now, she has helped taking care of them, and this has helped others in the lab with their research projects.

But now, Evelyn is starting a project of her own. Her soft voice betrays the nascent excitement, as she examines a well plate full of what looks like crusty, white dust.

“These are calcium carbonate crystals, and they look so beautiful under the microscope”, she says.

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On February 12, Evelyn Lewis examines test plates of CaCO3 precipitates in the lab

Thanks to a new supply grant from UConn’s Office for Undergraduate research, she will now have the opportunity to look at many more of these crystals. Evelyn’s research will focus on some of the smallest photosynthetic organisms in world, cyanobacteria. When they bloom they often coat themselves in slime that they can chemically manipulate. The conditions in this extracellular slime might then become favorable to bind carbon dioxide (CO2) in form of calcium carbonate (CaCO3), ultimately removing it from the atmosphere. In other words, cyanobacteria may be tiny but mighty as a natural tool for combating the increase of heat-trapping CO2 in the atmosphere.

“These natural options of using microbial slime for CO2 removal remain surprisingly underexplored”, explains Visscher. “The slime binds calcium and when it sinks to the bottom, it supports CaCO3 formation in sediments for thousands of years. This recently discovered mechanism provides novel insights into the global carbon cycle.”

So over the course of the next months, Evelyn will culture cyanobacteria again – but this time for her project. In small well plates, she will measure their CaCO3 production for about two weeks in relation to differing amounts of calcium. Yet the arguably coolest part will come after that, when the collected crystals will be examined using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).

Ultimately, the gathered data will allow testing the overarching hypothesis that the presence of cyanobacteria increases CaCO3 precipitation.

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SEM photograph of rhomboid CaCO3 crystals formed in the presence of a large amount of calcium (lots of slime)

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Needle-shaped carbonate crystals form when a smaller amount of calcium, or less slime, is present (note the difference in scale).

At DMS phytoplankton are now on IFCB-TV

The team of DMS researchers Dr. Zofia Baumann, Dr. Kate Randolph and Hazel Levine are happy to share that a major new instrument has begun its long anticipated work. The Imaging Flow Cytobot - or IFCB for short - is for now installed in the Rankin Seawater lab, after being purchased with a UConn-CLAS shared equipment grant nearly two years ago (Dierssen, Baumann et al.).

The instrument has the capacity to monitor and display in real time the breath-taking diversity of microscopic life in the ocean. Our IFCB focuses on the smaller size classes 5 - 150 um, which mostly represent single cell algae and small mixotrophs.

Leveraging additional NSF support, we were able to overcome challenges with operating the IFCB on a routine basis. The IFCB now accesses the intake line of the Rankin Lab (a very small fraction of it) and then photographs any particles and characteristic shapes. The compilation below shows a given size range to illustrate some of the diversity. The IFCB now records these images and displays them on a public-facing online Dashboard, which can be mesmerizing to watch.

 

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The composition of some of the larger phytoplankton as captured by the IFCB on February 6th 2025.

The implementation of the IFCB in Rankin Lab was led by Kate Randolph and greatly supported by Hazel Levine, Bob Dziomba, Charlie Woods, Todd Fake, and Chris Mills! Thank you.

The next step is to develop an AI-based classification system for automatic species identification. This will still take time, but we are collaborating with other IFCB users, including its inventors, and are optimistic about the progress ahead.

We hope you enjoy the stunning images of phytoplankton on what we like to call

"IFCB TV" !

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Dr. Randolph assembling the brand new IFCB in February of 2023. Photo credit: Dr. Zofia Baumann.

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Some of the DMS researchers (Dr. Zofia Baumann, Bridget Holohan, and Dr. Kate Randolph) attending the IFCB training at McLane Labs in February of 2023. Photo credit: Dr. Paola Batta-Lona