Ann Bucklin

My research focus is the molecular ecology and evolution of zooplankton. My laboratory group uses diverse molecular approaches to study population ecology, population genetics, phylogeography, species diversity, and evolution of marine holozooplankton, especially copepods and euphausiids.

Environmental Transcriptomics: We use genomic and transcriptomic approaches to examine gene expression profiles in zooplankton using whole-transcriptome RNA sequencing and quantitative real-time PCR (QPCR) of target biomarker genes. Our current focus includes the copepods Calanus finmarchicus in the North Atlantic and C. glacialis in the Arctic Ocean, as well as the salp Salpa thompsoni in the Southern Ocean.  For more about our studies on the Southern Ocean salp and Antarctic krill studies, see http://antarcticsalpgenomics.blogspot.com/.

Population Genetics / Phylogeography: We are using various genetic markers (including multigene sequences, Single Nucleotide Polymorphisms (SNPs), and Restriction-site Associated DNA (RAD) markers) to examine spatial patterns of genetic variation of zooplankton from small-scale patches to ocean-basin scale gyres, to measure population connectivity, and to infer pathways of transport in ocean currents.

Species biodiversity: We have worked to develop a new global view of zooplankton species diversity through the Census of Marine Zooplankton (CMarZ, see www.CMarZ.org), an ocean realm field project of the Census of Marine Life (CoML).  We use DNA sequences for mitochondrial cytochrome oxidase I as barcodes (i.e., short DNA sequences for species recognition and discrimination) to characterize global-scale patterns of zooplankton species distribution and diversity. We are analyzing zooplankton samples by metabarcoding, by High-Throughput Sequencing (e.g., Illumina MiSeq and NextSeq) of selected marker genes (COI and V4 and V9 hypervariable regions of the 18S ribosomal RNA (rRNA) gene of DNA extracted from unsorted zooplankton samples.  We are working to ensure that metabarcoding can become a reliable, accurate, rapid and cost-effective approach to assessment of species diversity and food web dynamics of pelagic ecosystems.  See https://theconversation.com/scientists-are-using-dna-to-study-ocean-life-and-reveal-the-hidden-diversity-of-zooplankton-97815 and  https://www.youtube.com/watch?v=A2–xV5eaB0

Phylogeny: We are using multigene datasets to analyze the evolutionary history and molecular phylogeny of copepods and euphausiids. A particular goal is to integrate molecular and morphological phylogenies for improved understanding of the timing and mode of speciation of these ecologically important and taxonomically complex zooplankton groups.

Research Projects

Environmental Genomics of Salpa thompsoni in the Southern Ocean – National Science Foundation / Office of Polar Programs

Strategic Initiative − The Arctic Ocean Ecosystem (SI_ARCTIC) – Norwegian Institute of Marine Research (Bergen and Tromsoe, Norway)

Chukchi Borderlands: Exploration of Pelagic Life in a Complex Polar Environment – NOAA Office of Ocean Exploration and Research

Expedition ANTARCTIC BLANC, in collaboration with Ocean Genome Legacy (https://www.northeastern.edu/ogl/). More information about the Expedition at http://antarcticblanc.com/about

Research Group Members and Visitors

Heidi D. Yeh (M.S. Student)

– Hayley DeHart (Research Technician) Email hayley.mills.dehart@gmail.com

– Paola Batta-Lona (Assistant Research Scientist) Email paola.batta_lona@uconn.edu

– Jennifer Questel (Postdoctoral Fellow, 2016-2017). Now at University of Alaska Fairbanks; Email jmquestel@alaska.edu

– Birgitte Nilsson (Visiting Graduate Student 2016-2017, Roskilde University, Denmark)

– Temur Shvelidze (Visiting Scientist 2018, Ilia State University, Tbilisi, Georgia) https://www.researchgate.net/profile/Temo_Shvelidze

Teaching

Marine Biology (MARN 3014) This course seeks to provide understanding of and appreciation for the diversity of marine life and the fundamental characteristics of marine environments. An important focus of the course is marine conservation biology, with guest lectures by Mystic Aquarium staff on endangered species, marine animal stranding, pollution, sound in the ocean, and other topics. (UConn – Avery Point; Spring semesters; Even years).  See https://magazine.uconn.edu/2018/05/17/marine-biology-marn-3014/

Seminar in Marine Biodiversity and Conservation (MARN 3812/ 5812) Co-taught with Dr. Tracy Romano. Presentations from guest experts and class discussions allow critical examination of state-of-the-art research, policy and regulatory frameworks of marine conservation biology and associated environmental, cultural, and socio-economic implications. (UConn – Avery Point; Spring Semesters; Odd Years)

Oceanography Alumni

– Kayla Erikson – M.S. 2015

– Paola Batta Lona – Ph.D. 2014

– Ebru Unal – Ph.D. 2011

– Brian Ortman – Ph.D. 2008

Honors Received

Fulbright Senior Fellowship, Institute of Marine Research, Bergen, Norway (1993-1994)

Fellow of the American Association for the Advancement of Science (Elected 1995)

Member, Connecticut Academy of Science and Engineering (Elected 2006)

International Council for Exploration of the Sea (ICES) Service Award (2017)  https://marinesciences.uconn.edu/2018/04/19/ices-service-award-to-ann-bucklin/

Publications (2010-2018)

For full reference list, see my ORCID page http://orcid.org/0000-0003-3750-9348 and Google Scholar Profile at https://scholar.google.com/citations?user=LHN47rkAAAAJ&hl=en&oi=ao

2018

Bucklin, A. K. DiVito, I. Smolina, M. Choquet, J.M. Questel, G. Hoarau and R.J. O’Neill (2018) Population Genomics of Marine Zooplankton. In: Population Genomics: Marine Organisms, O.P. Rajora and M. Oleksiak (Eds.), Springer Publ.  https://doi.org/10.1007/13836_2017_9.  Manuscript as submitted available at: https://darchive.mblwhoilibrary.org/handle/1912/9562

Nilsson, B., P.M. Jepsen, A. Bucklin and B.W. Hansen (2018) Environmental stress responses of marine copepods: Evidence of experimental handling artifacts for Acartia tonsa (Dana). Frontiers Mar. Science: doi:10.3389/fmars.2018.00156

2017

Batta-Lona, P.G., A. Maas, R. O’Neill, P.H. Wiebe, and A. Bucklin. 2017. Transcriptomic profiles of spring and summer populations of the Southern Ocean salp, Salpa thompsoni, in the Western Antarctic Peninsula region. Polar Biol. 40: 1261–1276. doi:10.1007/s00300-016-2051-6

Choquet, M., M. Hatlebakk, A.K.S. Dhanasiri, K. Kosobokova, I. Smolina, J.E. Søreide, C. Svensen, W. Melle, S. Kwasniewski, K. Eiane, M. Daase,V. Tverberg, S. Skreslet, A. Bucklin and G. Hoarau. 2017. Genetics redraws pelagic biogeography of Calanus. Biol.  Lett. 13: 20170588. http://dx.doi.org/10.1098/rsbl.2017.0588

Snelgrove, P., E. Vanden Berghe, P. Miloslavich, N. Bailly, A. Brandt, A. Bucklin, and 23 others. 2017. Global patterns in marine biodiversity (Chapter 34). In The First Global Integrated Marine Assessment: World Ocean Assessment I.  Cambridge University Press. Pp. 501-524. https://doi.org/10.1017/9781108186148.037

Wiebe, P.H., M.C. Benfield, and A. Bucklin. 2017. Sampling, preservation, and counting of samples: ii) Zooplankton, Chapter 10: In: Marine Plankton: A Practical Guide to Ecology, Methodology, and Taxonomy. Claudia Castellani and Martin Edwards [Eds.] Oxford University Press.

2016

Bucklin, A., P.K. Lindeque, N. Rodriguez-Ezpeleta, A. Albaina, and M. Lehtiniemi. 2016. Metabarcoding of marine zooplankton: Progress, prospects and pitfalls.  J. Plankton Res. Horizons 38: 393-400.  doi:10.1093/plankt/fbw023

Blanco-Bercial, L. and A. Bucklin. 2016. New view of population genetics of zooplankton: RAD-seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus. Molec. Ecol. 25: 1566-1580  doi: 10.1111/mec.13581

Jue, N., P.G. Batta-Lona, S. Trusiak, C. Obergfell, A. Bucklin, M. O’Neill, and R.J. O’Neill. 2016. Rapid evolutionary rates and unique genomic signatures discovered in the first reference genome for the Southern Ocean salp, Salpa thompsoni (Urochordata, Thaliacea). Genome Biol. Evol. 8: 3171-3186 doi: 10.1093/gbe/evw215

Nigro, L.M., M.V. Angel, K. Blachowiak-Samolyk, R.R. Hopcroft, and A. Bucklin. 2016. Identification, discrimination, and discovery of species of marine planktonic ostracods using DNA barcodes. PLoS-One 11(1): e0146327.  doi:10.1371/journal.pone.0146327

Questel, J.M., L. Blanco-Bercial, R.R. Hopcroft, and A. Bucklin. 2016. Phylogeography and connectivity of four sibling species of Pseudocalanus (Copepoda: Calanoida) in the North Pacific and Arctic Ocean. J. Plankton Res. doi:10.1093/plankt/fbw025

Wiebe, P.H., A. Bucklin, S. Kaartvedt, A. Røstad, and L. Blanco-Bercial. 2016. Vertical distribution and migration of euphausiid species in the Red Sea. J. Plankton Res.  doi:10.1093/plankt/fbw038

2015

Bucklin, A., D.J. McGillicuddy, P.H. Wiebe and C.S. Davis. 2015. Habitat usage by the cryptic copepods Pseudocalanus moultoni and P. newmani on Georges Bank (Northwest Atlantic). Cont. Shelf Res. 111: 83-94. doi:10.1016/j.csr.2015.11.001

Nair, V.R., F.X. Kidangan, R.G. Prabhu, A. Bucklin and S. Nair. 2015. DNA barcode of Chaetognatha from Indian Waters. Indian J. Geo-Marine Sci. 44: 1366-1376. http://nopr.niscair.res.in/handle/123456789/34924

Viñas, M.D., L. Blanco-Bercial, A. Bucklin, H. Verheye, J. Guilherme, F. Bersano, and S. Ceballos. 2015. Phylogeography of the copepod Calanoides carinatus s.l. (Krøyer) reveals cryptic species and delimits C. carinatus s.s. distribution in SW Atlantic Ocean. J. Exp. Mar. Biol. Ecol. 468: 97-104. doi:10.1016/j.jembe.2015.03.012

2014

Aarbakke, O.N., A. Bucklin, C. Halsband, and F. Norrbin. 2014. Comparative phylogeography and demographic history of five sibling species of Pseudocalanus (Copepoda: Calanoida) in the North Atlantic Ocean. J. Exp. Mar. Biol. Ecol. 461: 479-488. doi:10.1016/j.jembe.2014.10.006

Blanco-Bercial, L., A. Cornils, N.J. Copley, and A. Bucklin. 2014. DNA barcoding of marine copepods: assessment of analytical approaches to species identification. PLOS-Currents Tree of Life [2014 June 23]. doi: 10.1371/currents.tol.cdf8b74881f87e3b01d56b43791626d2

2013

Christie, A.E., V. Roncalli, P. Batta-Lona, M.D. McCoole, B.L. King, A. Bucklin, D.K. Hartline, and P.H. Lenz. 2013. In silico characterization of the insect diapause-associated protein couch potato (CPO) in Calanus finmarchicus (Crustacea: Copepoda). Comp. Biochem. Physiol. Part D 8: 45–57. doi:10.1016/j.cbd.2012.11.002

Unal, E., A. Bucklin, P.H. Lenz, and D.W. Towle. 2013. Gene expression of the marine copepod Calanus finmarchicus: Responses to small-scale environmental variation in the Gulf of Maine (NW Atlantic Ocean). J. exp. Mar. Biol. Ecol. 446:76-85. doi:10.1016/j.jembe.2013.04.020

2012

Cepeda, G.D., L. Blanco-Bercial, A. Bucklin, C.M. Berón, M.D. Viñas. 2012. Molecular taxonomic analysis of three species of Oithona (Copepoda, Cyclopoida) from the Argentine sector of the South Atlantic Ocean: comparative analysis using 28S rDNA. PLoS-One 7 (4): e35861. doi:10.1371/journal.pone.0035861

Kozol, R., L. Blanco-Bercial, and A. Bucklin. 2012. Lack of genetic divergence between Calanus agulhensis and C. sinicus (Copepoda; Calanoida). PLoS-One 7(10): e45710. doi:10.1371/journal.pone.0045710

Lenz, P.H., E. Unal, R.P. Hassett, C.M. Smith, A. Bucklin, A.E. Christie, and D.W. Towle. 2012. Functional genomics resources for the North Atlantic copepod, Calanus finmarchicus: EST database and physiological microarray. Comp. Biochem. Physiol. Part D, 7: 110–123. doi:10.1016/j.cbd.2011.12.001

2011

Aarbakke, O.N.S., A. Bucklin, C. Halsband-Lenk, and M.F. Norrbin. 2011. Discovery of Pseudocalanus moultoni Frost 1989 in Northeast Atlantic waters based on mitochondrial COI sequence variation. J. Plankton Res.   33:1487-1495. doi: 10.1093/plankt/fbr057

Batta-Lona, P.G., A. Bucklin, T. Patarnello, N.J. Copley, and P.H. Wiebe, 2011. Population genetic variation of the southern ocean krill, Euphausia superba, in the western Antarctic Peninsula region using Single Nucleotide Polymorphisms (SNPs).   Deep-Sea Res. II 58:1652-1661 doi:10.1016/j.dsr2.2010.11.017

Blanco-Bercial, L. F. álvarez-Marqués, and A. Bucklin. 2011. Comparative phylogeography and connectivity of sibling species of the marine copepod Clausocalanus (Calanoida). J. Exp. Mar. Biol. Ecol. 404: 108-115  doi:10.1016/j.jembe.2011.05.011

Blanco-Bercial, L., J. Bradford-Grieve, and A.Bucklin. 2011. Molecular phylogeny of Calanoida (Copepoda). Mol. Phylogen. Evol. 59:103–113 [doi.org/10.1016/j.ympev.2011.01.008 ]

Bortolotto, E., A. Bucklin, M. Mazzavilaa, L. Zane, and T. Patarnello. 2011. Gone with the currents: lack of genetic differentiation at the circum-continental scale in the Antarctic krill Euphausia superba. BMC – Genetics 12: 32  doi:10.1186/1471-2156-12-32

Bucklin, A., D. Steinke, and L. Blanco-Bercial, 2011. DNA barcoding of marine metazoa. Annual Review of Marine Science 2011, Vol. 3: 471–508 doi:10.1146/annurev-marine-120308-080950

Govindarajan, A.F., A. Bucklin, and L.P. Madin. 2011. A molecular phylogeny of the Thaliacea. J. Plankton Res. 33:843-853 doi:10.1093/plankt/fbq157

2010

Bucklin, A., R.R. Hopcroft, K.N. Kosobokova, L.M. Nigro, B.D. Ortman, R.M. Jennings, and C.J. Sweetman. 2010. DNA barcoding of Arctic Ocean holozooplankton for species identification and recognition.  Deep-Sea Res. II 57:40-48. doi:10.1016/j.dsr2.2009.08.005

Bucklin, A., S. Nishida, S. Schnack-Schiel, P.H. Wiebe, D. Lindsay, R.J. Machida, and N.J. Copley. 2010. Chapter 13. A Census of Zooplankton of the Global Ocean, In: Marine Life: Diversity, Distribution, and Abundance. A. McIntyre [Ed.], Wiley-Blackwell, Oxford.  Pp. 247-265

Bucklin, A., B.D. Ortman, R.M. Jennings, L. Nigro, C.J. Sweetman, N.J. Copley, and P.H. Wiebe. 2010. A “Rosetta Stone” for zooplankton: DNA barcode analysis of holozooplankton diversity of the Sargasso Sea (NW Atlantic Ocean). Deep-Sea Res. II 57:2234-2247. doi:10.1016/j.dsr2.2010.09.025

Grabbert, S., J. Renz, H.-J. Hirche, and A. Bucklin. 2010. Species-specific PCR (SS-PCR) discrimination of species of the calanoid copepod Pseudocalanus, P. acuspes and P. elongatus, in the Baltic and North Seas. Hydrobiologia 652:289-297. doi:10.1007/s10750-010-0360-2

Jennings, R.M., A. Bucklin, H. Ossenbrügger, and R.R. Hopcroft. 2010. Analysis of genetic diversity of planktonic gastropods from several ocean regions using DNA barcodes. Deep-Sea Research II 57:2199-2210. doi:10.1016/j.dsr2.2010.09.022

Jennings, R.M., A. Bucklin, and A.C. Pierrot-Bults. 2010. Barcoding of arrow worms (Phylum Chaetognatha) from three oceans: genetic diversity and evolution within an enigmatic phylum. PLoS-ONE. doi:10.1371/journal.pone.0009949

Ortman, B. D., A. Bucklin, F. Pages, and M. Youngbluth. 2010. DNA barcoding of the Medusozoa. Deep-Sea Res. II 57:2148-2156. doi:10.1016/j.dsr2.2010.09.017

Sutton, T.T., P.H. Wiebe, L.P. Madin and A. Bucklin. 2010. Diversity and community structure of pelagic fishes to 5000 m depth in the Sargasso Sea. Deep-Sea Res. II 57:2220-2233. doi:10.1016/j.dsr2.2010.09.024

Unal, E. and A. Bucklin. 2010. Basin-scale population genetic structure of the planktonic copepod Calanus finmarchicus in the North Atlantic Ocean. Prog. Oceanogr. 87:175-186. doi:10.1016/j.pocean.2010.09.017

Wiebe, P.H., A. Bucklin, L.P. Madin, M.V. Angel, T. Sutton, F. Pagés, R.R. Hopcroft, and D. Lindsay. 2010. Deep-sea holozooplankton species diversity in the Sargasso Sea, Northwestern Atlantic Ocean. Deep-Sea Res. II 57:2157-2166. doi:10.1016/j.dsr2.2010.09.018